東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁 到查詢結果 [ null ]

切換: 標籤 | MARC模式 | ISBD

PGE2 Signaling Triggers PECAM-Indepe...

Hayashi, Vanessa Sayuri,

FindBook

Google Book

Amazon

博客來

PGE2 Signaling Triggers PECAM-Independent Transendothelial Migration In Vitro and In Vivo /

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	PGE2 Signaling Triggers PECAM-Independent Transendothelial Migration In Vitro and In Vivo // Vanessa Sayuri Hayashi.
作者:	Hayashi, Vanessa Sayuri,
面頁冊數:	1 electronic resource (137 pages)
附註:	Source: Dissertations Abstracts International, Volume: 86-12, Section: B.
Contained By:	Dissertations Abstracts International86-12B.
標題:	Pathology. -
電子資源:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=32038002
ISBN:	9798315797739

PGE2 Signaling Triggers PECAM-Independent Transendothelial Migration In Vitro and In Vivo /
Hayashi, Vanessa Sayuri,

PGE2 Signaling Triggers PECAM-Independent Transendothelial Migration In Vitro and In Vivo /Vanessa Sayuri Hayashi. - 1 electronic resource (137 pages)

Source: Dissertations Abstracts International, Volume: 86-12, Section: B.

Genetic deletion or antibody blockade of platelet endothelial cell adhesion molecule-1 (PECAM, CD31) inhibits transendothelial migration (TEM) of leukocytes in all mouse strains studied except C57BL/6. Previously, we showed that this phenotype maps to a single 35.8 Mb locus on mouse chromosome 2, that contains the genes Ptgs1, Ptges, and Ptges2, which encode key enzymes involved in the Prostaglandin E2 (PGE2) synthesis pathway. PGE2 is a pro-inflammatory lipid mediator that binds four E prostanoid receptors (EP1-4). We hypothesized that PGE2 signaling supports TEM via a PECAM-independent mechanism. In vitro TEM assays demonstrate that PGE2 or 16,16-dimethyl PGE2 can restore transmigration of polymorphonuclear leukocytes (PMNs) and peripheral blood mononuclear cells (PBMCs) despite a TEM blockade with anti-PECAM antibody. This pro-transmigratory effect could be blocked with a combination of EP1 and EP3 antagonists, SC-51089 and DG-041, or with transient receptor potential canonical 6 (TRPC6) antagonist, BI-749327. 17-phenyl trinor PGE2, an agonist of EP1 and EP3, also restored transmigration of PMNs blocked with anti-PECAM antibody. In vivo, PGE2 overcame an anti-PECAM blockade when administered to FVB/n mice in thioglycolate peritonitis or croton oil dermatitis models, whereas blocking EP1 with SC-51089 decreased TEM in C57BL/6 PECAM-/- mice. Our findings demonstrate that PGE2 induces of PECAM-independent TEM, and pinpoint EP1 and EP3 as the receptors that relay said signal.

English

ISBN: 9798315797739Subjects--Topical Terms:

643180
Pathology.
Subjects--Index Terms:

Genetic deletion

PGE2 Signaling Triggers PECAM-Independent Transendothelial Migration In Vitro and In Vivo /
LDR:03081nmm a22004573i 4500 001 2436609
005 20251023121256.5
006 m o d
007 cr|nu||||||||
008 261011s2025 miu||||||m |||||||eng d
020 $a 9798315797739
035 $a (MiAaPQD)AAI32038002
035 $a AAI32038002
040 $a MiAaPQD $b eng $c MiAaPQD $e rda
100 1 $a Hayashi, Vanessa Sayuri, $e author. $0 (orcid)0000-0001-6968-1463 $3 3827337
245 1 0 $a PGE2 Signaling Triggers PECAM-Independent Transendothelial Migration In Vitro and In Vivo / $c Vanessa Sayuri Hayashi.
264 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2025
300 $a 1 electronic resource (137 pages)
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
500 $a Source: Dissertations Abstracts International, Volume: 86-12, Section: B.
500 $a Advisors: Muller, William A.; Green, Kathleen J. Committee members: Iruela-Arispe, Luisa M.; Sumagin, Ronen.
502 $b Ph.D. $c Northwestern University $d 2025.
520 $a Genetic deletion or antibody blockade of platelet endothelial cell adhesion molecule-1 (PECAM, CD31) inhibits transendothelial migration (TEM) of leukocytes in all mouse strains studied except C57BL/6. Previously, we showed that this phenotype maps to a single 35.8 Mb locus on mouse chromosome 2, that contains the genes Ptgs1, Ptges, and Ptges2, which encode key enzymes involved in the Prostaglandin E2 (PGE2) synthesis pathway. PGE2 is a pro-inflammatory lipid mediator that binds four E prostanoid receptors (EP1-4). We hypothesized that PGE2 signaling supports TEM via a PECAM-independent mechanism. In vitro TEM assays demonstrate that PGE2 or 16,16-dimethyl PGE2 can restore transmigration of polymorphonuclear leukocytes (PMNs) and peripheral blood mononuclear cells (PBMCs) despite a TEM blockade with anti-PECAM antibody. This pro-transmigratory effect could be blocked with a combination of EP1 and EP3 antagonists, SC-51089 and DG-041, or with transient receptor potential canonical 6 (TRPC6) antagonist, BI-749327. 17-phenyl trinor PGE2, an agonist of EP1 and EP3, also restored transmigration of PMNs blocked with anti-PECAM antibody. In vivo, PGE2 overcame an anti-PECAM blockade when administered to FVB/n mice in thioglycolate peritonitis or croton oil dermatitis models, whereas blocking EP1 with SC-51089 decreased TEM in C57BL/6 PECAM-/- mice. Our findings demonstrate that PGE2 induces of PECAM-independent TEM, and pinpoint EP1 and EP3 as the receptors that relay said signal.
546 $a English
590 $a School code: 0163
650 4 $a Pathology. $3 643180
650 4 $a Immunology. $3 611031
650 4 $a Cellular biology. $3 3172791
650 4 $a Genetics. $3 530508
653 $a Genetic deletion
653 $a Transendothelial migration
653 $a PECAM-independent mechanism
653 $a TRPC6 antagonist
653 $a EP3 antagonists
690 $a 0571
690 $a 0982
690 $a 0379
690 $a 0369
710 2 $a Northwestern University. $b Driskill Graduate Training Program in Life Sciences. $e degree granting institution. $3 3827178
720 1 $a Muller, William A. $e degree supervisor.
720 1 $a Green, Kathleen J. $e degree supervisor.
773 0 $t Dissertations Abstracts International $g 86-12B.
790 $a 0163
791 $a Ph.D.
792 $a 2025
856 4 0 $u https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=32038002