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Evaluation of Enumeration Methods fo...

Dhatt, Ujjalpreet Kaur,

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Evaluation of Enumeration Methods for Campylobacter and Salmonella in Poultry /

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Evaluation of Enumeration Methods for Campylobacter and Salmonella in Poultry // Ujjalpreet Kaur Dhatt.
作者:	Dhatt, Ujjalpreet Kaur,
面頁冊數:	1 electronic resource (106 pages)
附註:	Source: Masters Abstracts International, Volume: 86-07.
Contained By:	Masters Abstracts International86-07.
標題:	Food science. -
電子資源:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=31632903
ISBN:	9798346894438

Evaluation of Enumeration Methods for Campylobacter and Salmonella in Poultry /
Dhatt, Ujjalpreet Kaur,

Evaluation of Enumeration Methods for Campylobacter and Salmonella in Poultry /Ujjalpreet Kaur Dhatt. - 1 electronic resource (106 pages)

Source: Masters Abstracts International, Volume: 86-07.

Controlling Salmonella and Campylobacter in poultry requires robust quantification methods. This study compares enumeration methods in ground turkey, ground chicken, and chicken wings at varied inoculum levels (~8 log CFU/mL for high and ~5 log CFU/mL for low-level inoculation). Salmonella was quantified using conventional plating, miniaturized most probable number, and two PCR-based quantification assays. For Campylobacter, conventional plating, automated MPN, and PCR-based quantification assays were tested. ANOVA and Tukey's test revealed at high inoculation levels for Salmonella, mMPN resulted in the highest levels (p ≤ 0.05), followed by PCR assay 1, plating, and PCR assay 2. At low levels, mMPN, PCR Assay 1 & 2 performed similarly (p > 0.05) with plating significantly lower. For Campylobacter, the plating was higher than automated MPN at high levels (p ≤ 0.05), but PCR assay was significantly higher than both plating and automated MPN (p <.0001) at low levels. Traditional methods are reliable at higher contamination levels, whereas PCR-based precision is at low contamination levels.

English

ISBN: 9798346894438Subjects--Topical Terms:

3173303
Food science.
Subjects--Index Terms:

Campylobacter

Evaluation of Enumeration Methods for Campylobacter and Salmonella in Poultry /
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520 $a Controlling Salmonella and Campylobacter in poultry requires robust quantification methods. This study compares enumeration methods in ground turkey, ground chicken, and chicken wings at varied inoculum levels (~8 log CFU/mL for high and ~5 log CFU/mL for low-level inoculation). Salmonella was quantified using conventional plating, miniaturized most probable number, and two PCR-based quantification assays. For Campylobacter, conventional plating, automated MPN, and PCR-based quantification assays were tested. ANOVA and Tukey's test revealed at high inoculation levels for Salmonella, mMPN resulted in the highest levels (p ≤ 0.05), followed by PCR assay 1, plating, and PCR assay 2. At low levels, mMPN, PCR Assay 1 & 2 performed similarly (p > 0.05) with plating significantly lower. For Campylobacter, the plating was higher than automated MPN at high levels (p ≤ 0.05), but PCR assay was significantly higher than both plating and automated MPN (p <.0001) at low levels. Traditional methods are reliable at higher contamination levels, whereas PCR-based precision is at low contamination levels.
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650 4 $a Microbiology. $3 536250
650 4 $a Public health. $3 534748
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653 $a Most probable number
653 $a Prevalence
653 $a Salmonella
653 $a Quantification methods
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