東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁 到查詢結果 [ null ]

切換: 標籤 | MARC模式 | ISBD

Analysis of Fusarium oxysporum effec...

Simbaqueba, Jaime,

FindBook

Google Book

Amazon

博客來

Analysis of Fusarium oxysporum effectors shared between strains that infect cape gooseberry and tomato /

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Analysis of Fusarium oxysporum effectors shared between strains that infect cape gooseberry and tomato // Jaime Simbaqueba.
作者:	Simbaqueba, Jaime,
面頁冊數:	1 electronic resource (147 pages)
附註:	Source: Dissertations Abstracts International, Volume: 79-11, Section: C.
Contained By:	Dissertations Abstracts International79-11C.
標題:	Plant sciences. -
電子資源:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10820268
ISBN:	9781083488268

Analysis of Fusarium oxysporum effectors shared between strains that infect cape gooseberry and tomato /
Simbaqueba, Jaime,

Analysis of Fusarium oxysporum effectors shared between strains that infect cape gooseberry and tomato /Jaime Simbaqueba. - 1 electronic resource (147 pages)

Source: Dissertations Abstracts International, Volume: 79-11, Section: C.

Physalis peruviana, known as cape gooseberry, is a solanaceous plant native to tropical South America, typically growing in the Andes at 2000 m. Its economic value has grown due to its nutritional and medicinal properties. However, a vascular wilt disease caused by a newly discovered forma specialis of Fusarium oxysporum, (here designated f. sp. physali [Foph]), has become one of the limiting factors in cape gooseberry production, with losses up to 90%. The F. oxysporum species complex incudes numerous formae speciales (special forms), which are the causal agents of vascular wilt disease in a broad range of plants, including economically important crops such as banana, cotton, melon, tomato and recently, cape gooseberry. F. oxysporum f. sp. lycopersici (Fol) causes wilt disease on tomato. At least fourteen small secreted in xylem (SIX) proteins have been identified from the xylem sap of Fol-infected tomato plants. Five are associated with virulence and three are recognised by resistance proteins in the host. However, the function of most of these SIX proteins remains unclear. In this project, six homologues of Fol SIX genes (SIX1a, SIX1b, SIX7, SIX10, SIX12 and SIX15) and a homologue of Ave1 (an avirulence gene present in the broad-host-range wilt-pathogen Verticillium dahliae, with homologues in many other phytopathogens including Fol), were identified in Foph. These and other candidate effector genes were identified by mapping Foph RNAseq data against the Fol lineage-specific transcriptome and candidate F. oxysporum effector genes identified in other formae speciales in other studies. The Foph SIX gene and Ave1 homologues were found to encode proteins with 70 to 100% identity to their Fol counterparts, with the latter suggesting recent horizontal transfer of a cluster of SIX genes comprising SIX7, SIX10, SIX12 and SIX15. The Foph SIX1a and SIX1b proteins are 74% and 80% identical, respectively, to their Fol counterpart. Both homologues were tested in a ΔSIX1 strain of Fol to see if they could complement the virulence function of Fol SIX1 in tomato. The results showed no restoration of virulence for ten SIX1a and six SIX1b transformants tested, suggesting that their function might be restricted to cape gooseberry pathogenicity. Foph SIX1a and SIX1b transformants were also tested to see if they might be recognised by tomato plants carrying the I-3 resistance gene, which enables recognition of Fol SIX1 (i.e. Avr3). The results indicated that SIX1a was not recognised while SIX1b was recognised, suggesting that Foph-SIX1b may be recognised by I-3 as an avirulence factor and that the I-3 resistance gene could potentially be used in cape gooseberry plants to mediate Foph resistance. To investigate the function of SIX7, SIX10 and SIX12, a triple gene knockout strategy was initiated to assess their role in Fol virulence. This strategy included the use of the HSVtk (Herpes Simplex Virus thymidine kinase) gene as a counter selection marker against the ectopic insertion of transfer DNA (T-DNA) during fungal transformation by Agrobacterium tumefaciens. However, after several transformation attempts no gene knockouts were obtained. Attempts to produce single (SIX10) and double (SIX7 and SIX12) knockouts also failed. The Fol Rapid Alkalinisation Factor (RALF) gene was also subjected to gene disruption using this approach. Four RALF knockouts were obtained out of 44 transformants thereby validating the gene deletion strategy described above. Pathogenicity tests in tomato showed that these four mutants all developed disease symptoms that were not significantly different from those of wild type Fol under the assay conditions used.

English

ISBN: 9781083488268Subjects--Topical Terms:

3173832
Plant sciences.
Subjects--Index Terms:

Vascular wilt disease

Analysis of Fusarium oxysporum effectors shared between strains that infect cape gooseberry and tomato /
LDR:05153nmm a22004093i 4500 001 2430856
005 20250605050525.5
006 m o d
007 cr|nu||||||||
008 261011s2017 miu||||||m |||||||eng d
020 $a 9781083488268
035 $a (MiAaPQD)AAI10820268
035 $a (MiAaPQD)AustNatlU1885118320
035 $a AAI10820268
040 $a MiAaPQD $b eng $c MiAaPQD $e rda
100 1 $a Simbaqueba, Jaime, $e author. $3 3819384
245 1 0 $a Analysis of Fusarium oxysporum effectors shared between strains that infect cape gooseberry and tomato / $c Jaime Simbaqueba.
264 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2017
300 $a 1 electronic resource (147 pages)
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
500 $a Source: Dissertations Abstracts International, Volume: 79-11, Section: C.
500 $a Publisher info.: Dissertation/Thesis.
500 $a Advisors: Jones, David; Catanzariti, Ann Maree.
502 $b Ph.D. $c The Australian National University (Australia) $d 2017.
520 $a Physalis peruviana, known as cape gooseberry, is a solanaceous plant native to tropical South America, typically growing in the Andes at 2000 m. Its economic value has grown due to its nutritional and medicinal properties. However, a vascular wilt disease caused by a newly discovered forma specialis of Fusarium oxysporum, (here designated f. sp. physali [Foph]), has become one of the limiting factors in cape gooseberry production, with losses up to 90%. The F. oxysporum species complex incudes numerous formae speciales (special forms), which are the causal agents of vascular wilt disease in a broad range of plants, including economically important crops such as banana, cotton, melon, tomato and recently, cape gooseberry. F. oxysporum f. sp. lycopersici (Fol) causes wilt disease on tomato. At least fourteen small secreted in xylem (SIX) proteins have been identified from the xylem sap of Fol-infected tomato plants. Five are associated with virulence and three are recognised by resistance proteins in the host. However, the function of most of these SIX proteins remains unclear. In this project, six homologues of Fol SIX genes (SIX1a, SIX1b, SIX7, SIX10, SIX12 and SIX15) and a homologue of Ave1 (an avirulence gene present in the broad-host-range wilt-pathogen Verticillium dahliae, with homologues in many other phytopathogens including Fol), were identified in Foph. These and other candidate effector genes were identified by mapping Foph RNAseq data against the Fol lineage-specific transcriptome and candidate F. oxysporum effector genes identified in other formae speciales in other studies. The Foph SIX gene and Ave1 homologues were found to encode proteins with 70 to 100% identity to their Fol counterparts, with the latter suggesting recent horizontal transfer of a cluster of SIX genes comprising SIX7, SIX10, SIX12 and SIX15. The Foph SIX1a and SIX1b proteins are 74% and 80% identical, respectively, to their Fol counterpart. Both homologues were tested in a ΔSIX1 strain of Fol to see if they could complement the virulence function of Fol SIX1 in tomato. The results showed no restoration of virulence for ten SIX1a and six SIX1b transformants tested, suggesting that their function might be restricted to cape gooseberry pathogenicity. Foph SIX1a and SIX1b transformants were also tested to see if they might be recognised by tomato plants carrying the I-3 resistance gene, which enables recognition of Fol SIX1 (i.e. Avr3). The results indicated that SIX1a was not recognised while SIX1b was recognised, suggesting that Foph-SIX1b may be recognised by I-3 as an avirulence factor and that the I-3 resistance gene could potentially be used in cape gooseberry plants to mediate Foph resistance. To investigate the function of SIX7, SIX10 and SIX12, a triple gene knockout strategy was initiated to assess their role in Fol virulence. This strategy included the use of the HSVtk (Herpes Simplex Virus thymidine kinase) gene as a counter selection marker against the ectopic insertion of transfer DNA (T-DNA) during fungal transformation by Agrobacterium tumefaciens. However, after several transformation attempts no gene knockouts were obtained. Attempts to produce single (SIX10) and double (SIX7 and SIX12) knockouts also failed. The Fol Rapid Alkalinisation Factor (RALF) gene was also subjected to gene disruption using this approach. Four RALF knockouts were obtained out of 44 transformants thereby validating the gene deletion strategy described above. Pathogenicity tests in tomato showed that these four mutants all developed disease symptoms that were not significantly different from those of wild type Fol under the assay conditions used.
546 $a English
590 $a School code: 0433
650 4 $a Plant sciences. $3 3173832
650 4 $a Plant pathology. $3 3174872
653 $a Vascular wilt disease
690 $a 0479
690 $a 0480
710 2 $a The Australian National University (Australia). $e degree granting institution. $3 3765800
720 1 $a Jones, David $e degree supervisor.
720 1 $a Catanzariti, Ann Maree $e degree supervisor.
773 0 $t Dissertations Abstracts International $g 79-11C.
790 $a 0433
791 $a Ph.D.
792 $a 2017
856 4 0 $u https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10820268