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Engineering the Microenvironment at the Protein-Hydrogel Interface to Investigate the Role of the Extracellular Matrix Protein Type in Single Cell Cardiomyocyte Structure and Function.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Engineering the Microenvironment at the Protein-Hydrogel Interface to Investigate the Role of the Extracellular Matrix Protein Type in Single Cell Cardiomyocyte Structure and Function./
Author:	Castillo, Erica Araceli.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2021,
Description:	237 p.
Notes:	Source: Dissertations Abstracts International, Volume: 83-05, Section: B.
Contained By:	Dissertations Abstracts International83-05B.
Subject:	Reagents. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28812924
ISBN:	9798494454942

Engineering the Microenvironment at the Protein-Hydrogel Interface to Investigate the Role of the Extracellular Matrix Protein Type in Single Cell Cardiomyocyte Structure and Function.
Castillo, Erica Araceli.

Engineering the Microenvironment at the Protein-Hydrogel Interface to Investigate the Role of the Extracellular Matrix Protein Type in Single Cell Cardiomyocyte Structure and Function. - Ann Arbor : ProQuest Dissertations & Theses, 2021 - 237 p.

Source: Dissertations Abstracts International, Volume: 83-05, Section: B.

Thesis (Ph.D.)--Stanford University, 2021.

This item must not be sold to any third party vendors.

I developed hydrogel platforms to investigate the role of the extracellular matrix (ECM) proteins on cardiomyocyte structure and function. The biochemical and biophysical properties of the microenvironment are known to play a key role in cell structure and function. Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) hold great potential as a model to expand our knowledge of human heart muscle cells and their interactions with the surrounding microenvironment. In this thesis, I provide an introduction to cardiomyocytes and role of microenvironment properties in modulating cardiomyocyte structure and function. I present the gap in technologies that have been used to manipulate the protein-hydrogel interface (Chapter 1). I provide an in-depth review of the changes of native cardiomyocyte adhesion molecules and ECM composition throughout cardiac development and disease (Chapter 2). I provide an in-depth review of in vitro platforms for cardiomyocyte structural and function assessment (Chapter 3). I discuss the development of our protocol which consists of combination of lift-off protein patterning, a covalent protein-hydrogel linker, and the copolymerization protein transfer technique. We show that we are able to tune the ECM protein type across a wide variety of proteins including a mixture of ECM proteins, individual proteins, engineered biomaterials, and cell-cell adhesion proteins (Chapter 4). Next, I present traction force microscopy data comparing the hiPSC-CM morphology and contraction force on laminin versus fibronectin, with Matrigel as a positive control (Chapter 5). Last, I provide a summary of my findings, future platform development areas, and discuss future research directions my PhD work enables (Chapter 6). I provide ideas for how my hydrogel platforms could be implemented to study cardiac disease with interstitial fibrosis or cardiomyocytes with mutations in the cell adhesion molecules.

ISBN: 9798494454942Subjects--Topical Terms:

3683752
Reagents.

Engineering the Microenvironment at the Protein-Hydrogel Interface to Investigate the Role of the Extracellular Matrix Protein Type in Single Cell Cardiomyocyte Structure and Function.
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500 $a Source: Dissertations Abstracts International, Volume: 83-05, Section: B.
500 $a Advisor: Kenny, Thomas;Heilshorn, Sarah;Pruitt, Beth.
502 $a Thesis (Ph.D.)--Stanford University, 2021.
506 $a This item must not be sold to any third party vendors.
520 $a I developed hydrogel platforms to investigate the role of the extracellular matrix (ECM) proteins on cardiomyocyte structure and function. The biochemical and biophysical properties of the microenvironment are known to play a key role in cell structure and function. Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) hold great potential as a model to expand our knowledge of human heart muscle cells and their interactions with the surrounding microenvironment. In this thesis, I provide an introduction to cardiomyocytes and role of microenvironment properties in modulating cardiomyocyte structure and function. I present the gap in technologies that have been used to manipulate the protein-hydrogel interface (Chapter 1). I provide an in-depth review of the changes of native cardiomyocyte adhesion molecules and ECM composition throughout cardiac development and disease (Chapter 2). I provide an in-depth review of in vitro platforms for cardiomyocyte structural and function assessment (Chapter 3). I discuss the development of our protocol which consists of combination of lift-off protein patterning, a covalent protein-hydrogel linker, and the copolymerization protein transfer technique. We show that we are able to tune the ECM protein type across a wide variety of proteins including a mixture of ECM proteins, individual proteins, engineered biomaterials, and cell-cell adhesion proteins (Chapter 4). Next, I present traction force microscopy data comparing the hiPSC-CM morphology and contraction force on laminin versus fibronectin, with Matrigel as a positive control (Chapter 5). Last, I provide a summary of my findings, future platform development areas, and discuss future research directions my PhD work enables (Chapter 6). I provide ideas for how my hydrogel platforms could be implemented to study cardiac disease with interstitial fibrosis or cardiomyocytes with mutations in the cell adhesion molecules.
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