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Labelling of Proteinaceous Binders i...

Ooi, Su Yin.

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Labelling of Proteinaceous Binders in Art.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Labelling of Proteinaceous Binders in Art./
Author:	Ooi, Su Yin.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2019,
Description:	147 p.
Notes:	Source: Dissertations Abstracts International, Volume: 81-11, Section: B.
Contained By:	Dissertations Abstracts International81-11B.
Subject:	Analytical chemistry. -
Online resource:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=27933677
ISBN:	9781658479851

Labelling of Proteinaceous Binders in Art.
Ooi, Su Yin.

Labelling of Proteinaceous Binders in Art. - Ann Arbor : ProQuest Dissertations & Theses, 2019 - 147 p.

Source: Dissertations Abstracts International, Volume: 81-11, Section: B.

Thesis (Ph.D.)--Universidade de Evora (Portugal), 2019.

This item must not be sold to any third party vendors.

Easel paintings are important Cultural Heritage assets with significant historic and cultural value. They usually possess a multi-tiered structure, composed of different layers, some of which may present protein binders. Proteins have been commonly used as paintings medium, adhesives and coating layers in easel paintings. Hence, their recognition is a crucial step for easel painting's conservation and restoration processes. The present work presents a novel fluorescent labelling methodology, using a coumarin derivative chromophore, C392STP (sodium (E/Z)-4-(4-(2-(6,7-dimethoxycoumarin-3-yl)vinyl)benzoyl)-2,3,5,6-tetrafluorobenzenesulfonate) as a fluorophore probe to bond proteinaceous binders used in paintings. The method was developed and optimized using commercial proteins and proteins extracted from hen's egg yolk and white, bovine milk, and rabbit skin. In order to mimic the real conditions, paint models of easel paintings have been prepared by mixing proteins such as ovalbumin, casein and rabbit glue with different pigments (lead white, chrome yellow and black bone) and the fluorescent labelling method was miniaturized and tested. The results revealed that proteins in concentration as low as 6.0 μg/ml could be detected. Finally, for validation methodology, real micro samples of easel paintings were analyzed. The extracted proteins were submitted to the fluorescent labelling method developed and clearly identified in electrophoretic profiles. The results evidence the applicability of this methodology as an effective and useful analytical tool for the identification of protein binders obtained from easel paintings and, possibly in other art work. Additionally, theoretical quantum chemical calculations based on the Density Functional Theory (DFT) and Time Dependent Density Functional Theory (TD-DFT) have been performed in the C392STP coumarin and in a related coumarin derivative ((E/Z)-4-(2-(6,7-dimethoxycoumarin-3-yl)vinyl)-N-propylbenzamide), that mimics the coumarin bonded to lysine. The calculations confirm the experimental trends in absorption wavelengths and are in good agreement with the experimental absorption spectra, providing a comprehensive characterization of the main spectral features of the studied compounds.

ISBN: 9781658479851Subjects--Topical Terms:

3168300
Analytical chemistry.
Subjects--Index Terms:

Art conservation

Labelling of Proteinaceous Binders in Art.
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020 $a 9781658479851
035 $a (MiAaPQ)AAI27933677
035 $a (MiAaPQ)Portugal1017425920
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100 1 $a Ooi, Su Yin. $3 3558511
245 1 0 $a Labelling of Proteinaceous Binders in Art.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2019
300 $a 147 p.
500 $a Source: Dissertations Abstracts International, Volume: 81-11, Section: B.
502 $a Thesis (Ph.D.)--Universidade de Evora (Portugal), 2019.
506 $a This item must not be sold to any third party vendors.
520 $a Easel paintings are important Cultural Heritage assets with significant historic and cultural value. They usually possess a multi-tiered structure, composed of different layers, some of which may present protein binders. Proteins have been commonly used as paintings medium, adhesives and coating layers in easel paintings. Hence, their recognition is a crucial step for easel painting's conservation and restoration processes. The present work presents a novel fluorescent labelling methodology, using a coumarin derivative chromophore, C392STP (sodium (E/Z)-4-(4-(2-(6,7-dimethoxycoumarin-3-yl)vinyl)benzoyl)-2,3,5,6-tetrafluorobenzenesulfonate) as a fluorophore probe to bond proteinaceous binders used in paintings. The method was developed and optimized using commercial proteins and proteins extracted from hen's egg yolk and white, bovine milk, and rabbit skin. In order to mimic the real conditions, paint models of easel paintings have been prepared by mixing proteins such as ovalbumin, casein and rabbit glue with different pigments (lead white, chrome yellow and black bone) and the fluorescent labelling method was miniaturized and tested. The results revealed that proteins in concentration as low as 6.0 μg/ml could be detected. Finally, for validation methodology, real micro samples of easel paintings were analyzed. The extracted proteins were submitted to the fluorescent labelling method developed and clearly identified in electrophoretic profiles. The results evidence the applicability of this methodology as an effective and useful analytical tool for the identification of protein binders obtained from easel paintings and, possibly in other art work. Additionally, theoretical quantum chemical calculations based on the Density Functional Theory (DFT) and Time Dependent Density Functional Theory (TD-DFT) have been performed in the C392STP coumarin and in a related coumarin derivative ((E/Z)-4-(2-(6,7-dimethoxycoumarin-3-yl)vinyl)-N-propylbenzamide), that mimics the coumarin bonded to lysine. The calculations confirm the experimental trends in absorption wavelengths and are in good agreement with the experimental absorption spectra, providing a comprehensive characterization of the main spectral features of the studied compounds.
520 $a Identificacao de ligantes proteicos em arte As pinturas de cavalete sao um componente importante do Patrimonio Cultural, com um significativo valor historico e cultural. Geralmente possuem uma estrutura composta por diferentes camadas, algumas das quais podem apresentar ligantes proteicos. As proteinas surgem geralmente em pinturas de cavalete como meio de suporte da pintura, adesivos e camadas de revestimento. A sua identificacao e, portanto, um passo crucial para os processos de conservacao e restauracao da pintura de cavalete. O presente trabalho apresenta uma nova metodologia de marcacao fluorescente, utilizando um cromoforo derivado da cumarina, C392STP ((E/Z)-4-(2-(6,7- dimetoxicoumarin-3-yl)vinil)-N-propilbenzamida) como sonda fluorescente para marcar os ligantes proteicos usados em pinturas. O metodo foi desenvolvido e otimizado utilizando proteinas comerciais e proteinas extraidas da gema e clara de ovo de galinha, de leite de bovino e de pele de coelho. Para simular as condicoes reais, foram preparados modelos de pintura de pinturas de cavalete, misturando-se proteinas como ovalbumina, caseina e cola de coelho, com diferentes pigmentos (branco de chumbo, amarelo de cromio e negro de osso) e o metodo de marcacao fluorescente foi miniaturizado e testado. Com base nos resultados obtidos, o metodo revelou-se capaz de detetar proteinas a concentracao tao baixa quanto 6,0 μg / ml. Finalmente, para validacao do metodo, foram analisadas micro amostras reais de pinturas de cavalete. As proteinas extraidas foram submetidas ao metodo de marcacao fluorescente desenvolvido, tendo sido claramente identificadas em perfis eletroforeticos. Os resultados evidenciam a aplicabilidade desta metodologia como uma ferramenta analitica eficaz e util para a identificacao de ligantes proteicos extraidos de pinturas de cavalete e, possivelmente, de outras obras de arte. Adicionalmente, foram realizados calculos quanticos baseados na Teoria Funcional da Densidade (DFT) e na Teoria do Funcional da Densidade Dependente do Tempo (TD-DFT) da cumarina C392STP de um derivado desta cumarina, ((E/Z)-N-propyl-4-(2-(6,7-dimethoxy-2-oxo-2H-chromen-3-yl)vinyl)benzamide)), que modela a cumarina ligada a lisina. Os calculos confirmam as tendencias experimentais observadas nos comprimentos de onda de absorcao e estao de acordo com os espectros de absorcao experimentais, fornecendo uma caracterizacao abrangente das principais caracteristicas espectrais dos compostos estudados.
590 $a School code: 7023.
650 4 $a Analytical chemistry. $3 3168300
650 4 $a Biochemistry. $3 518028
653 $a Art conservation
690 $a 0486
690 $a 0487
710 2 $a Universidade de Evora (Portugal). $3 3429042
773 0 $t Dissertations Abstracts International $g 81-11B.
790 $a 7023
791 $a Ph.D.
792 $a 2019
793 $a English
856 4 0 $u https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=27933677