東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁 到查詢結果 [ null ]

切換: 標籤 | MARC模式 | ISBD

Serotonin Modulates a Calcium-Driven...

Zahratka, Jeffrey A.

FindBook

Google Book

Amazon

博客來

Serotonin Modulates a Calcium-Driven Negative Feedback Loop in a C. elegans Nociceptor.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Serotonin Modulates a Calcium-Driven Negative Feedback Loop in a C. elegans Nociceptor./
Author:	Zahratka, Jeffrey A.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2015,
Description:	126 p.
Notes:	Source: Dissertation Abstracts International, Volume: 78-06(E), Section: B.
Contained By:	Dissertation Abstracts International78-06B(E).
Subject:	Neurobiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10145313
ISBN:	9781369008456

Serotonin Modulates a Calcium-Driven Negative Feedback Loop in a C. elegans Nociceptor.
Zahratka, Jeffrey A.

Serotonin Modulates a Calcium-Driven Negative Feedback Loop in a C. elegans Nociceptor. - Ann Arbor : ProQuest Dissertations & Theses, 2015 - 126 p.

Source: Dissertation Abstracts International, Volume: 78-06(E), Section: B.

Thesis (Ph.D.)--The University of Toledo, 2015.

Neuromodulation in sensory circuits is critical because it allows an organism to respond appropriately to a given stimulus. Sensory systems are modulated by monoamine neurotransmitters as well as neuropeptides, which act in concert to regulate sensory circuits to give rise to complex behavioral states. One common technique for studying sensory circuits is brain activity mapping, where circuits are probed with fluorescent indicators whose readouts are related directly to neuronal activity. In the present work, we focus on the modulation of a pair of sensory neurons, the ASHs, in the nematode Caenorhabditis elegans. ASHs are polymodal, nociceptive neurons that are extensively modulated by monoamines and neuropeptides. Using a combination of genetics, Ca2+ imaging, electrophysiology, and behavioral assays, we have identified a complex instance where the monoamine serotonin (5-HT) stimulates aversive behaviors and neuronal depolarization, but decreases sensory-evoked Ca2+ signals, indicating that the recorded Ca 2+ levels do not positively correlate with neuronal activity. Mechanistically, 5-HT is likely acting through the SER-5 receptor and Galphaq signaling in ASHs to downregulate Ca2+ directly by initiating a Ca2+-driven negative feedback loop targeting the L-type Ca 2+ channel EGL-19. Together, these studies reveal a complex inhibitory feedback mechanism for sensory modulation, and have broad implications for activity mapping of complex neural circuits.

ISBN: 9781369008456Subjects--Topical Terms:

588707
Neurobiology.

Serotonin Modulates a Calcium-Driven Negative Feedback Loop in a C. elegans Nociceptor.
LDR:02380nmm a2200289 4500 001 2124633
005 20171031101038.5
008 180830s2015 ||||||||||||||||| ||eng d
020 $a 9781369008456
035 $a (MiAaPQ)AAI10145313
035 $a AAI10145313
040 $a MiAaPQ $c MiAaPQ
100 1 $a Zahratka, Jeffrey A. $3 3286638
245 1 0 $a Serotonin Modulates a Calcium-Driven Negative Feedback Loop in a C. elegans Nociceptor.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2015
300 $a 126 p.
500 $a Source: Dissertation Abstracts International, Volume: 78-06(E), Section: B.
502 $a Thesis (Ph.D.)--The University of Toledo, 2015.
520 $a Neuromodulation in sensory circuits is critical because it allows an organism to respond appropriately to a given stimulus. Sensory systems are modulated by monoamine neurotransmitters as well as neuropeptides, which act in concert to regulate sensory circuits to give rise to complex behavioral states. One common technique for studying sensory circuits is brain activity mapping, where circuits are probed with fluorescent indicators whose readouts are related directly to neuronal activity. In the present work, we focus on the modulation of a pair of sensory neurons, the ASHs, in the nematode Caenorhabditis elegans. ASHs are polymodal, nociceptive neurons that are extensively modulated by monoamines and neuropeptides. Using a combination of genetics, Ca2+ imaging, electrophysiology, and behavioral assays, we have identified a complex instance where the monoamine serotonin (5-HT) stimulates aversive behaviors and neuronal depolarization, but decreases sensory-evoked Ca2+ signals, indicating that the recorded Ca 2+ levels do not positively correlate with neuronal activity. Mechanistically, 5-HT is likely acting through the SER-5 receptor and Galphaq signaling in ASHs to downregulate Ca2+ directly by initiating a Ca2+-driven negative feedback loop targeting the L-type Ca 2+ channel EGL-19. Together, these studies reveal a complex inhibitory feedback mechanism for sensory modulation, and have broad implications for activity mapping of complex neural circuits.
590 $a School code: 0232.
650 4 $a Neurobiology. $3 588707
650 4 $a Neurosciences. $3 588700
650 4 $a Biology. $3 522710
690 $a 0421
690 $a 0317
690 $a 0306
710 2 $a The University of Toledo. $b Biology. $3 3175866
773 0 $t Dissertation Abstracts International $g 78-06B(E).
790 $a 0232
791 $a Ph.D.
792 $a 2015
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10145313