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The selection, isolation, and charac...

Leckett, Blaine.

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The selection, isolation, and characterization of a Chinese hamster lung fibroblast cell line resistant to an insulin-diphtheria A-chain toxic conjugate molecule.

Record Type:	Electronic resources : Monograph/item
Title/Author:	The selection, isolation, and characterization of a Chinese hamster lung fibroblast cell line resistant to an insulin-diphtheria A-chain toxic conjugate molecule./
Author:	Leckett, Blaine.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 1992,
Description:	170 p.
Notes:	Source: Dissertation Abstracts International, Volume: 54-01, Section: B, page: 1600.
Contained By:	Dissertation Abstracts International54-01B.
Subject:	Medicine. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=NN74546
ISBN:	9780315745469

The selection, isolation, and characterization of a Chinese hamster lung fibroblast cell line resistant to an insulin-diphtheria A-chain toxic conjugate molecule.
Leckett, Blaine.

The selection, isolation, and characterization of a Chinese hamster lung fibroblast cell line resistant to an insulin-diphtheria A-chain toxic conjugate molecule. - Ann Arbor : ProQuest Dissertations & Theses, 1992 - 170 p.

Source: Dissertation Abstracts International, Volume: 54-01, Section: B, page: 1600.

Thesis (Ph.D.)--McGill University (Canada), 1992.

The objective of this study was to determine how alterations in the insulin-receptor trafficking pathway affect ligand-dependent cellular action in Chinese hamster lung fibroblasts. Cell lines altered in their trafficking pathway were selected using a toxic insulin-diphtheria A-chain molecule (DTaI) which specifically interacts with the insulin receptor. One cell variant (IV-Al-j) which exhibited decreased insulin binding and enhanced insulin degradation did not demonstrate insulin or IGF-1 dependent cell proliferation. IV-Al-j cells also failed to produce an insulin-stimulated tyrosine phosphorylated protein (pp175). IGF-1 binding, insulin-stimulated hexose uptake, epidermal growth factor and thrombin stimulated cell proliferation were unaltered in IV-Al-j cells. These results suggest that the insulin and IGF-1 receptor may share a common pathway post-ligand binding for cell proliferation which does not involve EGF or thrombin, and that enhanced insulin degradation and loss of pp175 may be responsible for the loss of insulin-stimulated cell proliferation.

ISBN: 9780315745469Subjects--Topical Terms:

641104
Medicine.

The selection, isolation, and characterization of a Chinese hamster lung fibroblast cell line resistant to an insulin-diphtheria A-chain toxic conjugate molecule.
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245 1 4 $a The selection, isolation, and characterization of a Chinese hamster lung fibroblast cell line resistant to an insulin-diphtheria A-chain toxic conjugate molecule.
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300 $a 170 p.
500 $a Source: Dissertation Abstracts International, Volume: 54-01, Section: B, page: 1600.
502 $a Thesis (Ph.D.)--McGill University (Canada), 1992.
520 $a The objective of this study was to determine how alterations in the insulin-receptor trafficking pathway affect ligand-dependent cellular action in Chinese hamster lung fibroblasts. Cell lines altered in their trafficking pathway were selected using a toxic insulin-diphtheria A-chain molecule (DTaI) which specifically interacts with the insulin receptor. One cell variant (IV-Al-j) which exhibited decreased insulin binding and enhanced insulin degradation did not demonstrate insulin or IGF-1 dependent cell proliferation. IV-Al-j cells also failed to produce an insulin-stimulated tyrosine phosphorylated protein (pp175). IGF-1 binding, insulin-stimulated hexose uptake, epidermal growth factor and thrombin stimulated cell proliferation were unaltered in IV-Al-j cells. These results suggest that the insulin and IGF-1 receptor may share a common pathway post-ligand binding for cell proliferation which does not involve EGF or thrombin, and that enhanced insulin degradation and loss of pp175 may be responsible for the loss of insulin-stimulated cell proliferation.
590 $a School code: 0781.
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