東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back to Search results for [ null ]

Switch To: Labeled | MARC Mode | ISBD

Metabolic Profiling of Primary and S...

Kaiser, Kayla Anne.

Linked to FindBook

Google Book

Amazon

博客來

Metabolic Profiling of Primary and Secondary Biosynthetic Pathways in Angiosperms: Comparative Metabonomics and Applications of Hyphenated LC-NMR and LC-MS.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Metabolic Profiling of Primary and Secondary Biosynthetic Pathways in Angiosperms: Comparative Metabonomics and Applications of Hyphenated LC-NMR and LC-MS./
Author:	Kaiser, Kayla Anne.
Description:	406 p.
Notes:	Source: Dissertation Abstracts International, Volume: 73-08(E), Section: B.
Contained By:	Dissertation Abstracts International73-08B(E).
Subject:	Analytical chemistry. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3503279
ISBN:	9781267268129

Metabolic Profiling of Primary and Secondary Biosynthetic Pathways in Angiosperms: Comparative Metabonomics and Applications of Hyphenated LC-NMR and LC-MS.
Kaiser, Kayla Anne.

Metabolic Profiling of Primary and Secondary Biosynthetic Pathways in Angiosperms: Comparative Metabonomics and Applications of Hyphenated LC-NMR and LC-MS. - 406 p.

Source: Dissertation Abstracts International, Volume: 73-08(E), Section: B.

Thesis (Ph.D.)--University of California, Riverside, 2012.

The goal of this dissertation was to advance plant metabolomics through optimization of biological experimental design, sampling and sample preparation, data acquisition and pre-processing, and multivariable data analysis. The analytical platform most employed for comparative metabonomics was nuclear magnetic resonance (NMR). Liquid-chromatography (LC) coupled to NMR and mass spectrometry (MS) extended metabolic profile coverage from primary into secondary metabolic pathways. Comparative profiling of tissue extracts by LC-MS and complementary analyses by NMR were performed to establish metabolite identity and quantify responses to low-oxygen stress using the model Angiosperm, Arabidopsis thaliana.

ISBN: 9781267268129Subjects--Topical Terms:

3168300
Analytical chemistry.

Metabolic Profiling of Primary and Secondary Biosynthetic Pathways in Angiosperms: Comparative Metabonomics and Applications of Hyphenated LC-NMR and LC-MS.
LDR:03781nmm a2200349 4500 001 2060719
005 20150924074403.5
008 170521s2012 ||||||||||||||||| ||eng d
020 $a 9781267268129
035 $a (MiAaPQ)AAI3503279
035 $a AAI3503279
040 $a MiAaPQ $c MiAaPQ
100 1 $a Kaiser, Kayla Anne. $3 3174896
245 1 0 $a Metabolic Profiling of Primary and Secondary Biosynthetic Pathways in Angiosperms: Comparative Metabonomics and Applications of Hyphenated LC-NMR and LC-MS.
300 $a 406 p.
500 $a Source: Dissertation Abstracts International, Volume: 73-08(E), Section: B.
500 $a Includes supplementary digital materials.
500 $a Adviser: Cynthia K. Larive.
502 $a Thesis (Ph.D.)--University of California, Riverside, 2012.
520 $a The goal of this dissertation was to advance plant metabolomics through optimization of biological experimental design, sampling and sample preparation, data acquisition and pre-processing, and multivariable data analysis. The analytical platform most employed for comparative metabonomics was nuclear magnetic resonance (NMR). Liquid-chromatography (LC) coupled to NMR and mass spectrometry (MS) extended metabolic profile coverage from primary into secondary metabolic pathways. Comparative profiling of tissue extracts by LC-MS and complementary analyses by NMR were performed to establish metabolite identity and quantify responses to low-oxygen stress using the model Angiosperm, Arabidopsis thaliana.
520 $a Angiosperms, or flowering plants, constitute the most diverse and numerous group of land plants, and the most commercially important, producing an estimated 200,000 small molecule metabolites through conserved primary metabolism and divergent secondary pathways. No single metabolic profiling experiment has been able to quantify metabolite abundance over the relevant dynamic range (spanning seven orders of magnitude) and across the necessary structural diversity (chemical space coverage exceeds all known synthetic molecules). Challenges in plant metabolic profiling also arise through inherent limitations in methods (availability of diverse solid-phases, solvents, pure standards), instrument performance (reproducibility, robustness, speed, sensitivity), and data management (pre-processing, modeling, data basing and sharing).
520 $a The field of plant metabolic profiling has grown steadily over the past two decades, although it is still considered an emerging technique. Comparison of methods for sample preparation provided a foundation for one-dimensional NMR-based comparative metabonomics. Signal overlap was alleviated by liquid-liquid extraction (LLE), solid-phase extraction (SPE), and liquid chromatography (LC). A combined approach involving ultraviolet-visible absorption spectroscopy (UV-Vis), NMR, exact mass MS, and tandem MS (MS/MS) was used for dereplication.
520 $a Results are presented illustrating the ability of one-dimensional NMR combined with multivariable data analysis (MVDA) to quantify responses of a model plant to a biological problem of fundamental and practical relevance: cellular respiration under oxygen-limited conditions. The suitability of LC-NMR and LC-MS for dereplication is highlighted. Profiling of secondary metabolites by UPLC-MS combined with MVDA was undertaken for comparison with NMR-based comparative metabonomics. Efforts toward quantitative targeted analyses, hurdles and solutions, are highlighted.
590 $a School code: 0032.
650 4 $a Analytical chemistry. $3 3168300
650 4 $a Biochemistry. $3 518028
650 4 $a Plant sciences. $3 3173832
650 4 $a Botany. $3 516217
690 $a 0486
690 $a 0487
690 $a 0479
690 $a 0309
710 2 $a University of California, Riverside. $b Chemistry. $3 1682435
773 0 $t Dissertation Abstracts International $g 73-08B(E).
790 $a 0032
791 $a Ph.D.
792 $a 2012
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3503279