東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back to Search results for [ null ]

Switch To: Labeled | MARC Mode | ISBD

Regulation of cell fate by the stem ...

Frum, Tristan.

Linked to FindBook

Google Book

Amazon

博客來

Regulation of cell fate by the stem cell factors Cdx2, Oct4 and Sox2 in the early mouse embryo.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Regulation of cell fate by the stem cell factors Cdx2, Oct4 and Sox2 in the early mouse embryo./
Author:	Frum, Tristan.
Description:	145 p.
Notes:	Source: Dissertation Abstracts International, Volume: 75-11(E), Section: B.
Contained By:	Dissertation Abstracts International75-11B(E).
Subject:	Developmental biology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3630680
ISBN:	9781321087413

Regulation of cell fate by the stem cell factors Cdx2, Oct4 and Sox2 in the early mouse embryo.
Frum, Tristan.

Regulation of cell fate by the stem cell factors Cdx2, Oct4 and Sox2 in the early mouse embryo. - 145 p.

Source: Dissertation Abstracts International, Volume: 75-11(E), Section: B.

Thesis (Ph.D.)--University of California, Santa Cruz, 2014.

This item must not be sold to any third party vendors.

Pluripotent cells have the potential to create any cell type, and therefore represent a source of healthy cells by which to replace diseased cells in regenerative therapies. Thus, understanding how pluripotent cells are established and regulated is a major milestone in developing safe and effective regenerative therapies. In the mammalian embryo pluripotent cells emerge through two rounds of cell fate decisions that in addition to establishing pluripotent cells, establish two differentiated cell lineages. The molecules and signaling pathways that regulate these cell fate decisions represent key players in the establishment and regulation of pluripotent cells. In this thesis, I use the mouse embryo as a system in which to gain insight into how pluripotency is established and regulated. Cdx2, Oct4 and Sox2 are transcription factors that are important regulators of pluripotency in vitro, but their role in regulating the cell fate decisions that establish pluripotency in vivo is less clear. By using a loss-of-function approach, I define the roles of each of these factors in regulating the cell fate decisions that establish pluripotency in the early mouse embryo. First, by eliminating maternal and zygotic Cdx2 I demonstrate that Cdx2 is not a maternal determinant for pluripotency or differentiation during early embryo cell fate decisions. Second, I eliminate both maternal and zygotic Oct4 and demonstrate that the initial specification of pluripotent and non-pluripotent lineages in the early mouse embryo is independent of Oct4, and that instead, Oct4 cell-autonomously regulates both formation of pluripotent cells, and differentiation of a non-pluripotent lineage, the primitive endoderm. Third, I demonstrate that in contrast to Oct4, Sox2 promotes differentiation of primitive endoderm non-cell autonomously. These results support the model that pluripotency is acquired through these early embryonic cell fate decisions, rather than being determined during early embryogenesis. Furthermore, analysis of the roles of Oct4 and Sox2 in these early embryonic cell fate decisions highlights distinct roles for these factors in establishing pluripotency and suggests that although in vitro Oct4 and Sox2 appear to have overlapping roles, these master regulators of pluripotency have distinct cell type specific roles in regulating the cell fate decisions that establish pluripotency in the early mouse embryo. Taken together, the research presented in this thesis greatly increases our understanding of the regulation of the cell fate decisions that establish pluripotency in the embryo and provides important insights into the biology of key regulators of pluripotency in vitro and in vivo..

ISBN: 9781321087413Subjects--Topical Terms:

592588
Developmental biology.

Regulation of cell fate by the stem cell factors Cdx2, Oct4 and Sox2 in the early mouse embryo.
LDR:03766nmm a2200301 4500 001 2059480
005 20150805065220.5
008 170521s2014 ||||||||||||||||| ||eng d
020 $a 9781321087413
035 $a (MiAaPQ)AAI3630680
035 $a AAI3630680
040 $a MiAaPQ $c MiAaPQ
100 1 $a Frum, Tristan. $3 3173557
245 1 0 $a Regulation of cell fate by the stem cell factors Cdx2, Oct4 and Sox2 in the early mouse embryo.
300 $a 145 p.
500 $a Source: Dissertation Abstracts International, Volume: 75-11(E), Section: B.
500 $a Adviser: Amy Ralston.
502 $a Thesis (Ph.D.)--University of California, Santa Cruz, 2014.
506 $a This item must not be sold to any third party vendors.
506 $a This item must not be added to any third party search indexes.
520 $a Pluripotent cells have the potential to create any cell type, and therefore represent a source of healthy cells by which to replace diseased cells in regenerative therapies. Thus, understanding how pluripotent cells are established and regulated is a major milestone in developing safe and effective regenerative therapies. In the mammalian embryo pluripotent cells emerge through two rounds of cell fate decisions that in addition to establishing pluripotent cells, establish two differentiated cell lineages. The molecules and signaling pathways that regulate these cell fate decisions represent key players in the establishment and regulation of pluripotent cells. In this thesis, I use the mouse embryo as a system in which to gain insight into how pluripotency is established and regulated. Cdx2, Oct4 and Sox2 are transcription factors that are important regulators of pluripotency in vitro, but their role in regulating the cell fate decisions that establish pluripotency in vivo is less clear. By using a loss-of-function approach, I define the roles of each of these factors in regulating the cell fate decisions that establish pluripotency in the early mouse embryo. First, by eliminating maternal and zygotic Cdx2 I demonstrate that Cdx2 is not a maternal determinant for pluripotency or differentiation during early embryo cell fate decisions. Second, I eliminate both maternal and zygotic Oct4 and demonstrate that the initial specification of pluripotent and non-pluripotent lineages in the early mouse embryo is independent of Oct4, and that instead, Oct4 cell-autonomously regulates both formation of pluripotent cells, and differentiation of a non-pluripotent lineage, the primitive endoderm. Third, I demonstrate that in contrast to Oct4, Sox2 promotes differentiation of primitive endoderm non-cell autonomously. These results support the model that pluripotency is acquired through these early embryonic cell fate decisions, rather than being determined during early embryogenesis. Furthermore, analysis of the roles of Oct4 and Sox2 in these early embryonic cell fate decisions highlights distinct roles for these factors in establishing pluripotency and suggests that although in vitro Oct4 and Sox2 appear to have overlapping roles, these master regulators of pluripotency have distinct cell type specific roles in regulating the cell fate decisions that establish pluripotency in the early mouse embryo. Taken together, the research presented in this thesis greatly increases our understanding of the regulation of the cell fate decisions that establish pluripotency in the embryo and provides important insights into the biology of key regulators of pluripotency in vitro and in vivo..
590 $a School code: 0036.
650 4 $a Developmental biology. $3 592588
650 4 $a Cellular biology. $3 3172791
690 $a 0758
690 $a 0379
710 2 $a University of California, Santa Cruz. $b Molecular Cell and Developmental Biology. $3 2101013
773 0 $t Dissertation Abstracts International $g 75-11B(E).
790 $a 0036
791 $a Ph.D.
792 $a 2014
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3630680