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Identification of Human Blood Messen...

Xu, Yanlin.

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Identification of Human Blood Messenger Ribonucleic Acids through Non-Polymerase Chain Reaction Based Multiplexing.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Identification of Human Blood Messenger Ribonucleic Acids through Non-Polymerase Chain Reaction Based Multiplexing./
Author:	Xu, Yanlin.
Description:	61 p.
Notes:	Source: Masters Abstracts International, Volume: 52-04.
Contained By:	Masters Abstracts International52-04(E).
Subject:	Biology, Molecular. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1550940
ISBN:	9781303672354

Identification of Human Blood Messenger Ribonucleic Acids through Non-Polymerase Chain Reaction Based Multiplexing.
Xu, Yanlin.

Identification of Human Blood Messenger Ribonucleic Acids through Non-Polymerase Chain Reaction Based Multiplexing. - 61 p.

Source: Masters Abstracts International, Volume: 52-04.

Thesis (M.S.)--University of Central Oklahoma, 2013.

The goal of this research project was to develop a method for the identification of human blood that was simple and fast to use, yet sensitive and specific enough for forensic casework. Based on these criteria, the ideal assay would be based on messenger ribonucleic acid (mRNA) multiplexing specific for blood identification. According to the central dogma of molecular biology and gene expression, it can be theorized that the identity of human specific biological material can be obtained based only on the mRNA expressed by genes of a particular tissue. Once isolated, the tissue specific mRNA can be detected using the affinity of deoxyribonucleic acid (DNA) probes which contain the complementary sequence for annealing. The newly annealed double stranded nucleic acid will then serve as the target for detection via fluorescent molecular labeling, which will allow the human specific biological material targets to be detectable under ultraviolet light. Development of such a method would provide the field with a more rapid and accurate assay for analysis of forensic serology samples.

ISBN: 9781303672354Subjects--Topical Terms:

1017719
Biology, Molecular.

Identification of Human Blood Messenger Ribonucleic Acids through Non-Polymerase Chain Reaction Based Multiplexing.
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100 1 $a Xu, Yanlin. $3 2104624
245 1 0 $a Identification of Human Blood Messenger Ribonucleic Acids through Non-Polymerase Chain Reaction Based Multiplexing.
300 $a 61 p.
500 $a Source: Masters Abstracts International, Volume: 52-04.
500 $a Adviser: Dwight Adams.
502 $a Thesis (M.S.)--University of Central Oklahoma, 2013.
520 $a The goal of this research project was to develop a method for the identification of human blood that was simple and fast to use, yet sensitive and specific enough for forensic casework. Based on these criteria, the ideal assay would be based on messenger ribonucleic acid (mRNA) multiplexing specific for blood identification. According to the central dogma of molecular biology and gene expression, it can be theorized that the identity of human specific biological material can be obtained based only on the mRNA expressed by genes of a particular tissue. Once isolated, the tissue specific mRNA can be detected using the affinity of deoxyribonucleic acid (DNA) probes which contain the complementary sequence for annealing. The newly annealed double stranded nucleic acid will then serve as the target for detection via fluorescent molecular labeling, which will allow the human specific biological material targets to be detectable under ultraviolet light. Development of such a method would provide the field with a more rapid and accurate assay for analysis of forensic serology samples.
590 $a School code: 0503.
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Chemistry, Biochemistry. $3 1017722
650 4 $a Chemistry, Analytical. $3 586156
690 $a 0307
690 $a 0487
690 $a 0486
710 2 $a University of Central Oklahoma. $b Forensic Science Institute. $3 2091923
773 0 $t Masters Abstracts International $g 52-04(E).
790 $a 0503
791 $a M.S.
792 $a 2013
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1550940