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Partial complementation of the UV se...

Yao, Hanlong.

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Partial complementation of the UV sensitivity of Deinococcus radiodurans excision repair mutants by the cloned denV gene of bacteriophage T4.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Partial complementation of the UV sensitivity of Deinococcus radiodurans excision repair mutants by the cloned denV gene of bacteriophage T4./
Author:	Yao, Hanlong.
Description:	74 p.
Notes:	Source: Masters Abstracts International, Volume: 29-01, page: 0117.
Contained By:	Masters Abstracts International29-01.
Subject:	Physics, Molecular. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1341150

Partial complementation of the UV sensitivity of Deinococcus radiodurans excision repair mutants by the cloned denV gene of bacteriophage T4.
Yao, Hanlong.

Partial complementation of the UV sensitivity of Deinococcus radiodurans excision repair mutants by the cloned denV gene of bacteriophage T4. - 74 p.

Source: Masters Abstracts International, Volume: 29-01, page: 0117.

Thesis (M.S.)--Uniformed Services University of the Health Sciences, 1990.

Deinococcus radiodurans has two endonucleases that incise UV irradiated DNA, UV endonuclease-Subjects--Topical Terms:

1018648
Physics, Molecular.

Partial complementation of the UV sensitivity of Deinococcus radiodurans excision repair mutants by the cloned denV gene of bacteriophage T4.
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005 20041215074551.5
008 130614s1990 eng d
035 $a (UnM)AAI1341150
035 $a AAI1341150
040 $a UnM $c UnM
100 1 $a Yao, Hanlong. $3 1949614
245 1 0 $a Partial complementation of the UV sensitivity of Deinococcus radiodurans excision repair mutants by the cloned denV gene of bacteriophage T4.
300 $a 74 p.
500 $a Source: Masters Abstracts International, Volume: 29-01, page: 0117.
500 $a Director: Kenneth W. Minton.
502 $a Thesis (M.S.)--Uniformed Services University of the Health Sciences, 1990.
520 $a Deinococcus radiodurans has two endonucleases that incise UV irradiated DNA, UV endonuclease- $\ alpha $ and UV endonuclease- $\ beta $, that are believed to functionally overlap. Both endonucleases must be mutationally inactivated to yield an incisionless, markedly UV-sensitive phenotype. denV, the bacteriophage T4 gene encoding pyrimidine dimer-DNA glycosylase (PD-glycosylase) was introduced and expressed via duplication insertion in D. radiodurans wild type, and single and double UV endonuclease mutants. Expression of denV increased UV survival of an incisionless double mutant and the strain deficient only in UV endonuclease- $\ beta $. The strain deficient in UV endonuclease- $\ alpha $ has wild-type UV-resistance, and the expression of PD-glycosylase exerted no survival effect on this strain or wild-type. These results suggest that UV endonuclease- $\ alpha $ does not recognize one or more types of cyclobutane dimer incised by the PD-glycosylase or UV endonuclease- $\ beta $.
590 $a School code: 0888.
650 4 $a Physics, Molecular. $3 1018648
690 $a 0609
710 2 0 $a Uniformed Services University of the Health Sciences. $3 1916055
773 0 $t Masters Abstracts International $g 29-01.
790 1 0 $a Minton, Kenneth W., $e advisor
790 $a 0888
791 $a M.S.
792 $a 1990
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1341150