東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back to Search results for [ null ]

Switch To: Labeled | MARC Mode | ISBD

Characterization of the glucose cata...

Zhang, Yongmei.

Linked to FindBook

Google Book

Amazon

博客來

Characterization of the glucose catalytic pathways in Deinococcus radiodurans.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Characterization of the glucose catalytic pathways in Deinococcus radiodurans./
Author:	Zhang, Yongmei.
Description:	136 p.
Notes:	Source: Dissertation Abstracts International, Volume: 61-11, Section: B, page: 5704.
Contained By:	Dissertation Abstracts International61-11B.
標題:	Biology, Microbiology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9993841
ISBN:	0493029494

Characterization of the glucose catalytic pathways in Deinococcus radiodurans.
Zhang, Yongmei.

Characterization of the glucose catalytic pathways in Deinococcus radiodurans. - 136 p.

Source: Dissertation Abstracts International, Volume: 61-11, Section: B, page: 5704.

Thesis (Ph.D.)--The University of Memphis, 2000.

Deinococcus radiodurans is very resistant to radiation and many chemicals that damage DNA. High-resolution factorial design models were used to analyze the effects of the nutrient interactions in the tryptone-glucose-yeast extract (TGY) growth medium on biomass formation. Results suggested that the cell mass was mainly provided by tryptone and yeast-extract, but not glucose, in the medium. In vivo analysis showed that cells growing on TGY medium utilized glucose by the pentose phosphate (PP) pathway. Mn could induce the Embden-Meyerhof-Parnas (EMP) pathway in D. radiodurans . The EMP pathway competed with the PP pathway for glucose, resulting in a rapid depletion of glucose in the medium. However, the Mn-induced EMP pathway was a futile pathway, i.e., this pathway did not promote biomass formation.

ISBN: 0493029494Subjects--Topical Terms:

1017734
Biology, Microbiology.

Characterization of the glucose catalytic pathways in Deinococcus radiodurans.
LDR:02696nmm 2200289 4500 001 1862012
005 20041215074547.5
008 130614s2000 eng d
020 $a 0493029494
035 $a (UnM)AAI9993841
035 $a AAI9993841
040 $a UnM $c UnM
100 1 $a Zhang, Yongmei. $3 1949583
245 1 0 $a Characterization of the glucose catalytic pathways in Deinococcus radiodurans.
300 $a 136 p.
500 $a Source: Dissertation Abstracts International, Volume: 61-11, Section: B, page: 5704.
500 $a Major Professor: Tit-Yee Wong.
502 $a Thesis (Ph.D.)--The University of Memphis, 2000.
520 $a Deinococcus radiodurans is very resistant to radiation and many chemicals that damage DNA. High-resolution factorial design models were used to analyze the effects of the nutrient interactions in the tryptone-glucose-yeast extract (TGY) growth medium on biomass formation. Results suggested that the cell mass was mainly provided by tryptone and yeast-extract, but not glucose, in the medium. In vivo analysis showed that cells growing on TGY medium utilized glucose by the pentose phosphate (PP) pathway. Mn could induce the Embden-Meyerhof-Parnas (EMP) pathway in D. radiodurans . The EMP pathway competed with the PP pathway for glucose, resulting in a rapid depletion of glucose in the medium. However, the Mn-induced EMP pathway was a futile pathway, i.e., this pathway did not promote biomass formation.
520 $a To confirm the above hypothesis, mutants defective in either the PP or the EMP pathway were generated. The marker enzyme for the PP pathway was the NADP+-dependent glucose-6-phosphate dehydrogenase ( zwf) and the marker enzyme for the EMP pathway was the fructose-1,6-bisphosphate aldolase (fda). Deinococcus radiodurans zwf - and fda- mutants were then tested for their resistance to UV irradiation, H2O2, methylmethanesalfonic acid (MMS) and mitomycin C (MMC).
520 $a The futile EMP pathway in D. radiodurans was further studied in vitro. Enzymatic analysis showed that glucose and Mn together could fully induce the aldolase activity in the cell-free extract of D. radiodurans. Glucose or Mn alone could only partially induce the enzyme. The amino acid sequence of the deinococcal aldolase shared significant homology with other known Class II aldolases. However, unlike most microbial aldolase, the deinococcal enzyme required Mn, not Zn, as the cofactor. (Abstract shortened by UMI.)
590 $a School code: 1194.
650 4 $a Biology, Microbiology. $3 1017734
690 $a 0410
710 2 0 $a The University of Memphis. $3 1025952
773 0 $t Dissertation Abstracts International $g 61-11B.
790 1 0 $a Wong, Tit-Yee, $e advisor
790 $a 1194
791 $a Ph.D.
792 $a 2000
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9993841