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An investigation of the application ...

Mann, Tina Louise.

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An investigation of the application of ultrasound as a rapid method to provide DNA fragments suitable for detection by DNA biosensors.

Record Type:	Electronic resources : Monograph/item
Title/Author:	An investigation of the application of ultrasound as a rapid method to provide DNA fragments suitable for detection by DNA biosensors./
Author:	Mann, Tina Louise.
Description:	118 p.
Notes:	Source: Masters Abstracts International, Volume: 42-03, page: 0940.
Contained By:	Masters Abstracts International42-03.
Subject:	Chemistry, Analytical. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=MQ84154
ISBN:	0612841545

An investigation of the application of ultrasound as a rapid method to provide DNA fragments suitable for detection by DNA biosensors.
Mann, Tina Louise.

An investigation of the application of ultrasound as a rapid method to provide DNA fragments suitable for detection by DNA biosensors. - 118 p.

Source: Masters Abstracts International, Volume: 42-03, page: 0940.

Thesis (M.Sc.)--University of Toronto (Canada), 2003.

Ultrasound exposure was examined as a method to cleave genomic DNA in a rapid manner. High frequency ultrasound of 20 kHz was applied to DNA solutions under a variety of experimental conditions. A Yates Pattern Analysis determined that ionic strength and the duration of sonication were the most significant factors impacting fragmentation patterns. A salt concentration of 1 M caused genomic DNA to resist fragmentation and fragment sizes were centred near 500 base pairs and larger. Decreasing the ionic strength to 0.l M NaCl or less resulted in the formation of fragment sizes close to 100 base pairs. Manipulation of these factors allowed for some control of fragmentation patterns. Hyperchromicity experiments, studies considering the influence of various gases, and simulations to determine the probability of destroying a target sequence were also done. Experiments suggested that ultrasound can be used as a rapid method to generate DNA fragments.

ISBN: 0612841545Subjects--Topical Terms:

586156
Chemistry, Analytical.

An investigation of the application of ultrasound as a rapid method to provide DNA fragments suitable for detection by DNA biosensors.
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245 1 3 $a An investigation of the application of ultrasound as a rapid method to provide DNA fragments suitable for detection by DNA biosensors.
300 $a 118 p.
500 $a Source: Masters Abstracts International, Volume: 42-03, page: 0940.
500 $a Adviser: Ulrich Krull.
502 $a Thesis (M.Sc.)--University of Toronto (Canada), 2003.
520 $a Ultrasound exposure was examined as a method to cleave genomic DNA in a rapid manner. High frequency ultrasound of 20 kHz was applied to DNA solutions under a variety of experimental conditions. A Yates Pattern Analysis determined that ionic strength and the duration of sonication were the most significant factors impacting fragmentation patterns. A salt concentration of 1 M caused genomic DNA to resist fragmentation and fragment sizes were centred near 500 base pairs and larger. Decreasing the ionic strength to 0.l M NaCl or less resulted in the formation of fragment sizes close to 100 base pairs. Manipulation of these factors allowed for some control of fragmentation patterns. Hyperchromicity experiments, studies considering the influence of various gases, and simulations to determine the probability of destroying a target sequence were also done. Experiments suggested that ultrasound can be used as a rapid method to generate DNA fragments.
590 $a School code: 0779.
650 4 $a Chemistry, Analytical. $3 586156
650 4 $a Chemistry, Biochemistry. $3 1017722
690 $a 0486
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