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Development of mass spectrometric te...

Guo, Nan.

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Development of mass spectrometric techniques for protein sequencing and metabolome analysis.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Development of mass spectrometric techniques for protein sequencing and metabolome analysis./
Author:	Guo, Nan.
Description:	142 p.
Notes:	Source: Masters Abstracts International, Volume: 44-06, page: 2798.
Contained By:	Masters Abstracts International44-06.
Subject:	Chemistry, Analytical. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=MR13818
ISBN:	9780494138182

Development of mass spectrometric techniques for protein sequencing and metabolome analysis.
Guo, Nan.

Development of mass spectrometric techniques for protein sequencing and metabolome analysis. - 142 p.

Source: Masters Abstracts International, Volume: 44-06, page: 2798.

Thesis (M.Sc.)--University of Alberta (Canada), 2006.

The objective of this research is to develop new mass spectrometric methods for protein and metabolite characterization. One major challenge in protein characterization by mass spectrometry (MS) is the study of protein modifications. In this work, a technique involving gel electrophoresis separation of proteins, protein extraction, microwave-assisted acid hydrolysis of the extracted protein, and MS analysis of the polypeptides is developed. This technique is used to characterize a hemoglobin variant from a human blood sample. For human metabolite analysis, one major challenge is to identify the metabolites in a complex sample. A tandem MS technique is developed for metabolite identification based on the match of acquired MS/MS spectra with those in a MS/MS spectral library. The spectral library is created by using a triple quadrupole mass spectrometer and consists of 215 human metabolites. It is demonstrated that this technique can be used to identify metabolites in human urine.

ISBN: 9780494138182Subjects--Topical Terms:

586156
Chemistry, Analytical.

Development of mass spectrometric techniques for protein sequencing and metabolome analysis.
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035 $a (UnM)AAIMR13818
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100 1 $a Guo, Nan. $3 1920710
245 1 0 $a Development of mass spectrometric techniques for protein sequencing and metabolome analysis.
300 $a 142 p.
500 $a Source: Masters Abstracts International, Volume: 44-06, page: 2798.
502 $a Thesis (M.Sc.)--University of Alberta (Canada), 2006.
520 $a The objective of this research is to develop new mass spectrometric methods for protein and metabolite characterization. One major challenge in protein characterization by mass spectrometry (MS) is the study of protein modifications. In this work, a technique involving gel electrophoresis separation of proteins, protein extraction, microwave-assisted acid hydrolysis of the extracted protein, and MS analysis of the polypeptides is developed. This technique is used to characterize a hemoglobin variant from a human blood sample. For human metabolite analysis, one major challenge is to identify the metabolites in a complex sample. A tandem MS technique is developed for metabolite identification based on the match of acquired MS/MS spectra with those in a MS/MS spectral library. The spectral library is created by using a triple quadrupole mass spectrometer and consists of 215 human metabolites. It is demonstrated that this technique can be used to identify metabolites in human urine.
590 $a School code: 0351.
650 4 $a Chemistry, Analytical. $3 586156
650 4 $a Chemistry, Biochemistry. $3 1017722
690 $a 0486
690 $a 0487
710 2 0 $a University of Alberta (Canada). $3 626651
773 0 $t Masters Abstracts International $g 44-06.
790 $a 0351
791 $a M.Sc.
792 $a 2006
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=MR13818