東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back to Search results for [ null ]

Switch To: Labeled | MARC Mode | ISBD

Epitope specificity as a prime deter...

Kelly-Quintos, Casie Anne.

Linked to FindBook

Google Book

Amazon

博客來

Epitope specificity as a prime determinant of the opsonic and protective activity of human antibodies to the bacterial surface polysaccharide PNAG.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Epitope specificity as a prime determinant of the opsonic and protective activity of human antibodies to the bacterial surface polysaccharide PNAG./
Author:	Kelly-Quintos, Casie Anne.
Description:	125 p.
Notes:	Source: Dissertation Abstracts International, Volume: 67-05, Section: B, page: 2356.
Contained By:	Dissertation Abstracts International67-05B.
Subject:	Biology, Microbiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3217784
ISBN:	9780542693014

Epitope specificity as a prime determinant of the opsonic and protective activity of human antibodies to the bacterial surface polysaccharide PNAG.
Kelly-Quintos, Casie Anne.

Epitope specificity as a prime determinant of the opsonic and protective activity of human antibodies to the bacterial surface polysaccharide PNAG. - 125 p.

Source: Dissertation Abstracts International, Volume: 67-05, Section: B, page: 2356.

Thesis (Ph.D.)--Harvard University, 2006.

Poly N-Acetyl Glucosamine (PNAG) is a surface expressed polysaccharide that is a known virulence factor of Staphylococcus aureus and recently shown to be expressed by numerous pathogenic bacteria. PNAG was previously demonstrated to be the target of protective antibodies when used as a vaccine in animal studies; however, no reports of the human antibody response to PNAG have been published. This dissertation describes the importance of epitope specificity in the human immune response to PNAG. The PNAG molecule is N-acetylated and therefore this dissertation analyzes the contribution of both acetate dependant and acetate independent epitopes to the ability of antibodies specific to these epitopes to opsonize S. aureus. This dissertation demonstrates that antibodies specific to the deacetylated epitopes on PNAG have more opsonic and protective activity against S. aureus by using S. aureus colonized CF patient serum and human monoclonal antibodies (mAbs) specific to different epitopes on PNAG. A possible mechanism explaining the superiority of deacetylated PNAG (dPNAG) specific antibodies is shown with the elucidation of the function of the IcaB protein as a deacetylase that is required for the cell association of PNAG. Overall, this dissertation suggests that vaccination or treatment with dPNAG or dPNAG specific antibodies respectively will be the most effective means of targeting PNAG on staphylococcus or other PNAG expressing bacteria for opsonophagocytosis.

ISBN: 9780542693014Subjects--Topical Terms:

1017734
Biology, Microbiology.

Epitope specificity as a prime determinant of the opsonic and protective activity of human antibodies to the bacterial surface polysaccharide PNAG.
LDR:02416nmm 2200265 4500 001 1829038
005 20071106080109.5
008 130610s2006 eng d
020 $a 9780542693014
035 $a (UMI)AAI3217784
035 $a AAI3217784
040 $a UMI $c UMI
100 1 $a Kelly-Quintos, Casie Anne. $3 1917910
245 1 0 $a Epitope specificity as a prime determinant of the opsonic and protective activity of human antibodies to the bacterial surface polysaccharide PNAG.
300 $a 125 p.
500 $a Source: Dissertation Abstracts International, Volume: 67-05, Section: B, page: 2356.
500 $a Adviser: Gerald B. Pier.
502 $a Thesis (Ph.D.)--Harvard University, 2006.
520 $a Poly N-Acetyl Glucosamine (PNAG) is a surface expressed polysaccharide that is a known virulence factor of Staphylococcus aureus and recently shown to be expressed by numerous pathogenic bacteria. PNAG was previously demonstrated to be the target of protective antibodies when used as a vaccine in animal studies; however, no reports of the human antibody response to PNAG have been published. This dissertation describes the importance of epitope specificity in the human immune response to PNAG. The PNAG molecule is N-acetylated and therefore this dissertation analyzes the contribution of both acetate dependant and acetate independent epitopes to the ability of antibodies specific to these epitopes to opsonize S. aureus. This dissertation demonstrates that antibodies specific to the deacetylated epitopes on PNAG have more opsonic and protective activity against S. aureus by using S. aureus colonized CF patient serum and human monoclonal antibodies (mAbs) specific to different epitopes on PNAG. A possible mechanism explaining the superiority of deacetylated PNAG (dPNAG) specific antibodies is shown with the elucidation of the function of the IcaB protein as a deacetylase that is required for the cell association of PNAG. Overall, this dissertation suggests that vaccination or treatment with dPNAG or dPNAG specific antibodies respectively will be the most effective means of targeting PNAG on staphylococcus or other PNAG expressing bacteria for opsonophagocytosis.
590 $a School code: 0084.
650 4 $a Biology, Microbiology. $3 1017734
690 $a 0410
710 2 0 $a Harvard University. $3 528741
773 0 $t Dissertation Abstracts International $g 67-05B.
790 1 0 $a Pier, Gerald B., $e advisor
790 $a 0084
791 $a Ph.D.
792 $a 2006
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3217784