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Quantitative frequency-domain fluore...

Cerussi, Albert Edward.

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Quantitative frequency-domain fluorescence spectroscopy in tissues and tissue-like media.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Quantitative frequency-domain fluorescence spectroscopy in tissues and tissue-like media./
Author:	Cerussi, Albert Edward.
Description:	250 p.
Notes:	Source: Dissertation Abstracts International, Volume: 60-03, Section: B, page: 1145.
Contained By:	Dissertation Abstracts International60-03B.
Subject:	Physics, Optics. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9921670
ISBN:	0599209089

Quantitative frequency-domain fluorescence spectroscopy in tissues and tissue-like media.
Cerussi, Albert Edward.

Quantitative frequency-domain fluorescence spectroscopy in tissues and tissue-like media. - 250 p.

Source: Dissertation Abstracts International, Volume: 60-03, Section: B, page: 1145.

Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1999.

In the never-ending quest for improved medical technology at lower cost, modern near-infrared optical spectroscopy offers the possibility of inexpensive technology for quantitative and non-invasive diagnoses. Hemoglobin is the dominant chromophore in the 700--900 nm spectral region and as such it allows for the optical assessment of hemoglobin concentration and tissue oxygenation by absorption spectroscopy. However, there are many other important physiologically relevant compounds or physiological states that cannot be effectively sensed via optical methods because of poor optical contrast. In such cases, contrast enhancements are required.

ISBN: 0599209089Subjects--Topical Terms:

1018756
Physics, Optics.

Quantitative frequency-domain fluorescence spectroscopy in tissues and tissue-like media.
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100 1 $a Cerussi, Albert Edward. $3 1905217
245 1 0 $a Quantitative frequency-domain fluorescence spectroscopy in tissues and tissue-like media.
300 $a 250 p.
500 $a Source: Dissertation Abstracts International, Volume: 60-03, Section: B, page: 1145.
500 $a Adviser: Enrico Gratton.
502 $a Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1999.
520 $a In the never-ending quest for improved medical technology at lower cost, modern near-infrared optical spectroscopy offers the possibility of inexpensive technology for quantitative and non-invasive diagnoses. Hemoglobin is the dominant chromophore in the 700--900 nm spectral region and as such it allows for the optical assessment of hemoglobin concentration and tissue oxygenation by absorption spectroscopy. However, there are many other important physiologically relevant compounds or physiological states that cannot be effectively sensed via optical methods because of poor optical contrast. In such cases, contrast enhancements are required.
520 $a Fluorescence spectroscopy is an attractive component of optical tissue spectroscopy. Exogenous fluorophores, as well as some endogenous ones, may furnish the desperately needed sensitivity and specificity that is lacking in near-infrared optical tissue spectroscopy. The main focus of this thesis was to investigate the generation and propagation of fluorescence photons inside tissues and tissue-like media (i.e., scattering dominated media). The standard concepts of fluorescence spectroscopy have been incorporated into a diffusion-based picture that is sometimes referred to as photon migration. The novelty of this work lies in the successful quantitative recovery of fluorescence lifetimes, absolute fluorescence quantum yields, fluorophore concentrations, emission spectra, and both scattering and absorption coefficients at the emission wavelength from a tissue-like medium. All of these parameters are sensitive to the fluorophore local environment and hence are indicators of the tissue's physiological state.
520 $a One application demonstrating the capabilities of frequency-domain lifetime spectroscopy in tissue-like media is a study of the binding of ethidium bromide to bovine leukocytes in fresh milk. Ethidium bromide is a fluorescent dye that is commonly used to label DNA, and hence visualize chromosomes in cells. The lifetime of ethidium bromide increases by an order of magnitude upon binding to DNA. In this thesis, I demonstrated that the fluorescence photon migration model is capable of accurately determining the somatic cell count (SCC) in a milk sample. Although meant as a demonstration of fluorescence tissue spectroscopy, this specific problem has important implications for the dairy industry's warfare against subclinical mastitis (i.e., mammary gland inflammation), since the SCC is often used as an indication of bovine infection.
590 $a School code: 0090.
650 4 $a Physics, Optics. $3 1018756
650 4 $a Biophysics, Medical. $3 1017681
650 4 $a Chemistry, Analytical. $3 586156
690 $a 0752
690 $a 0760
690 $a 0486
710 2 0 $a University of Illinois at Urbana-Champaign. $3 626646
773 0 $t Dissertation Abstracts International $g 60-03B.
790 1 0 $a Gratton, Enrico, $e advisor
790 $a 0090
791 $a Ph.D.
792 $a 1999
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9921670