東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back to Search results for [ null ]

Switch To: Labeled | MARC Mode | ISBD

Synthetic peptide nano-1 increases t...

Chin, Shook-Fong.

Linked to FindBook

Google Book

Amazon

博客來

Synthetic peptide nano-1 increases the uptake of single-walled carbon nanotubes by HeLa cells.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Synthetic peptide nano-1 increases the uptake of single-walled carbon nanotubes by HeLa cells./
Author:	Chin, Shook-Fong.
Description:	171 p.
Notes:	Source: Dissertation Abstracts International, Volume: 66-05, Section: B, page: 2556.
Contained By:	Dissertation Abstracts International66-05B.
Subject:	Chemistry, Analytical. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3176113
ISBN:	9780542147012

Synthetic peptide nano-1 increases the uptake of single-walled carbon nanotubes by HeLa cells.
Chin, Shook-Fong.

Synthetic peptide nano-1 increases the uptake of single-walled carbon nanotubes by HeLa cells. - 171 p.

Source: Dissertation Abstracts International, Volume: 66-05, Section: B, page: 2556.

Thesis (Ph.D.)--The University of Texas at Dallas, 2005.

Numerous discoveries and procedures developed by the UTD BioNano Group with respect to the isolation and aqueous solubilization of single-walled carbon nanotubes have set the stage for their use in biomedical applications. The key ingredient in these works is a 29-amino acid residue peptide termed nano-1 that can coat carbon nanotubes. The work presented in this Fundamental Practicum describes the first experiments exposing mammalian cells to these coated nanotubes. First, the synthesis and characterization of dye-labeled nano-1 was achieved to permit confocal fluorescence microscopy of HeLa cells incubated with peptide-coated carbon nanotubes. Second, a modified sonication and centrifugation procedure was developed to enrich for shorter length (&sim;100-nm long) peptide-coated nanotubes to facilitate their entry into cells. Third, conditions whereby peptide, nanotubes, and peptide-coated nanotubes did not alter the growth characteristic of HeLa cells over the course of 4 days were determined. Fourth, Raman spectroscopy and confocal fluorescence microscopy were used to reveal that: (i) nanotubes (peptide-coated and non-coated) entered HeLa cells in a time- and metabolic-dependent fashion; (ii) that both peptide-coated and non-coated nanotubes could be detected in the cytoplasm and nucleus of cells; (iii) that higher relative ratios of uncoated nanotubes were distributed in the nucleus relative to the cytoplasm; (iv) that higher relative ratios of peptide-coated nanotubes were distributed in the cytoplasm relative to the nucleus; (v) that &sim;3-fold more peptide-coated nanotubes entered the nucleus of HeLa cells relative to the non-coated nanotubes; and finally, (vi) that &sim;8-fold more peptide-coated nanotubes entered the cytoplasm of HeLa cells relative to the non-coated nanotubes.

ISBN: 9780542147012Subjects--Topical Terms:

586156
Chemistry, Analytical.

Synthetic peptide nano-1 increases the uptake of single-walled carbon nanotubes by HeLa cells.
LDR:02709nam 2200265 4500 001 1395903
005 20110527105417.5
008 130515s2005 ||||||||||||||||| ||eng d
020 $a 9780542147012
035 $a (UMI)AAI3176113
035 $a AAI3176113
040 $a UMI $c UMI
100 1 $a Chin, Shook-Fong. $3 1674643
245 1 0 $a Synthetic peptide nano-1 increases the uptake of single-walled carbon nanotubes by HeLa cells.
300 $a 171 p.
500 $a Source: Dissertation Abstracts International, Volume: 66-05, Section: B, page: 2556.
500 $a Supervisor: Paul Pantano.
502 $a Thesis (Ph.D.)--The University of Texas at Dallas, 2005.
520 $a Numerous discoveries and procedures developed by the UTD BioNano Group with respect to the isolation and aqueous solubilization of single-walled carbon nanotubes have set the stage for their use in biomedical applications. The key ingredient in these works is a 29-amino acid residue peptide termed nano-1 that can coat carbon nanotubes. The work presented in this Fundamental Practicum describes the first experiments exposing mammalian cells to these coated nanotubes. First, the synthesis and characterization of dye-labeled nano-1 was achieved to permit confocal fluorescence microscopy of HeLa cells incubated with peptide-coated carbon nanotubes. Second, a modified sonication and centrifugation procedure was developed to enrich for shorter length (&sim;100-nm long) peptide-coated nanotubes to facilitate their entry into cells. Third, conditions whereby peptide, nanotubes, and peptide-coated nanotubes did not alter the growth characteristic of HeLa cells over the course of 4 days were determined. Fourth, Raman spectroscopy and confocal fluorescence microscopy were used to reveal that: (i) nanotubes (peptide-coated and non-coated) entered HeLa cells in a time- and metabolic-dependent fashion; (ii) that both peptide-coated and non-coated nanotubes could be detected in the cytoplasm and nucleus of cells; (iii) that higher relative ratios of uncoated nanotubes were distributed in the nucleus relative to the cytoplasm; (iv) that higher relative ratios of peptide-coated nanotubes were distributed in the cytoplasm relative to the nucleus; (v) that &sim;3-fold more peptide-coated nanotubes entered the nucleus of HeLa cells relative to the non-coated nanotubes; and finally, (vi) that &sim;8-fold more peptide-coated nanotubes entered the cytoplasm of HeLa cells relative to the non-coated nanotubes.
590 $a School code: 0382.
650 4 $a Chemistry, Analytical. $3 586156
690 $a 0486
710 2 $a The University of Texas at Dallas. $3 1018411
773 0 $t Dissertation Abstracts International $g 66-05B.
790 1 0 $a Pantano, Paul, $e advisor
790 $a 0382
791 $a Ph.D.
792 $a 2005
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3176113